Stepwise
Kirsten Gotting
Overview
Analyze the Mnemiopsis data with the new filtered transcriptome that Eric generated.
Spearman Correlation
The following plots the spearman correlation of the RPM (reads per million) for all genes that have an average RPM ≥ 1.
Spearman’s rank correlation coefficient is a nonparametric measure of rank correlation, recommended for data that doesn’t necessarily come from a bivariate normal distribution.
Pairwise PCA
MA and Volcano plots
MA plot: The log2 fold change(M) plotted against the log2 average(A) of the normalized read count for each gene. Significantly differentially expressed genes are plotted in red.
Volcano plot: The log2 fold change(M) plotted against the -log10 (eg. 1e^-10 = 10) of the adjusted p-value.
1hpf/2hpf
2hpf/3hpf
3hpf/4hpf
4hpf/5hpf
5hpf/6hpf
6hpf/7hpf
7hpf/8hpf
Heatmaps
The first heatmap was made by hierarchically clustering the euclidean distances of the log2 fold change for genes with p-values less than 10^{-5} at any time point.
The second heatmap uses the same hierarchical method to cluster timepoints.
---
title: "Stepwise" 
author: "Kirsten Gotting"
output:
  html_document:
      code_download: true
      toc: true
      toc_depth: 2
      toc_float:
        collapsed: false
---


```{r initializeDataStepwise, echo = FALSE, results = "hide", message = FALSE, error = FALSE, warning = FALSE, cache = FALSE}

## user input to 'DESeq2All.Rdata'

load("./output/DESeq2AllStepwise.Rdata")
setwd("./")

## First list the R libraries I want to use
libraries        <- c("DESeq2", "gplots", "RColorBrewer", 'knitr', 'grid', 'gridExtra', "tidyverse", 'kiRsten')

## Now read in the libraries
lapply(libraries, function(x){
    library(x, character.only = TRUE, quietly = TRUE)
    })

## Initialize knitr options
opts_chunk$set(echo=FALSE, message=FALSE, results="hide", fig.keep="all", warning=FALSE, error=FALSE, fig.path="figures/", cache = TRUE)
opts_knit$set(root.dir = "./")


```





# Overview

Analyze the Mnemiopsis data with the new filtered transcriptome that Eric generated.


#Spearman Correlation


The following plots the spearman correlation of the RPM (reads per million) for all genes that have an average RPM &#8805; 1.

Spearman's rank correlation coefficient is a nonparametric measure of rank correlation, recommended for data that doesn't necessarily come from a bivariate normal distribution.


```{r spearman_correlationStepwise, fig.cap = ""}

## Make a spearman plot


filteredRPMs <- normCounts %>% group_by(ID) %>% mutate(rpmFlag = ifelse((mean(rpm) >= 1), yes = 'yes', no = 'no')) %>% ungroup() %>%
    filter(rpmFlag == 'yes') %>% select(ID, Sample, rpm) %>% spread(Sample, rpm)



rpmfilt        <- data.frame(select(filteredRPMs, -ID), row.names = filteredRPMs$ID, check.names = FALSE)
spearman       <- data.frame(cor(rpmfilt, method = 'spearman'), check.names = FALSE)
spearman$ID    <- rownames(spearman)
spearmanTidy   <- spearman %>% gather(Sample, correlation, -ID)

## and plot it!

ggplot(data = spearmanTidy, aes(x = ID, y = Sample, fill = correlation)) + geom_tile() +
    scale_fill_gradient2(low = "yellow", mid = 'white', high = "purple4", midpoint = 0.8, limit = c(0,1)) +
        ggtitle('Spearman Correlation of Samples') + xlab('') + ylab('') +
            theme(axis.text.x = element_text(angle = 90, hjust = 1))
```


# Pairwise PCA

```{r PCAStepwise, fig.fullwidth = TRUE, fig.width = 10, fig.height = 10}

pcas_data <- lapply(contrast_names, function(x){
    y <- plotPCA(rld_all[[x]], returnData = TRUE, ntop = 10000)
    y$ID <- x
    y
})

pca_data <- bind_rows(pcas_data)

ggplot(data = pca_data, aes(x = PC1, y = PC2, label = name, colour = factor(condition))) +
            geom_point(size = 3) + theme_bw() + geom_text(hjust = 0, vjust = -0.4) +
            xlab("PC 1") + ylab("PC 2") + 
            scale_x_continuous(expand = c(0.98, 0))  +
            scale_y_continuous(expand = c(0.2, 0)) +
            theme(legend.position="none") +
            facet_wrap(~ ID, scales = "free")

```



# MA and Volcano plots {.tabset}

MA plot: The log2 fold change(M) plotted against the log2 average(A) of the normalized read count for each gene.  Significantly differentially expressed genes are plotted in red.

Volcano plot: The log2 fold change(M) plotted against the -log10 (eg. 1e^-10 = 10) of the adjusted p-value.

```{r mavolStepwise}


x <- contrast_names[[1]]

plots <- lapply(contrast_names, function(x){
    df      <- all_results_tidyDF %>% filter(contrastID == x) %>% spread(dea_ID, dea_Value) %>% mutate(logbaseMean = log(baseMean))
    pval    <- as.numeric(pval)
    if(is.null(df)){
        message(paste0("Pvalue too stringent for ", contrast, ". MA/Volcano plot ommited.\n"))
    } else {
        pval                  <- as.numeric(pval)
        lower_label           <- paste0("Genes with Counts: ", nrow(df), "\nUp: ",#create the label of the legend
                                  nrow(subset(df, log2FoldChange > 0 & padj < pval)),
                                  ", Down: ", nrow(subset(df, log2FoldChange < 0 & padj < pval)), "\n")
  
        df <- df %>% mutate(significance_group = ifelse(padj <= pval, yes = 'yes', no = 'no'))
  
        legend_labels        <- c(paste0("padj > ", pval,": ", nrow(filter(df, significance_group == 'no'))),
                                  paste0("padj < ", pval,": ", nrow(filter(df, significance_group == 'yes'))))
        ma.plot <-   ggplot(df, aes(x = logbaseMean, y = log2FoldChange, group = significance_group, colour = significance_group)) +
                      geom_point() + theme_bw() +
                      ggtitle('MA plot') + scale_x_continuous(expand = c(0, 0)) +
                      xlab("A = Mean of log2(Abundance)") + ylab("M = log2(Fold Change)") +
                      scale_color_manual(values = c("gray24", "red"), name = lower_label, breaks = c("gray24", "red"), labels = legend_labels) +
                      geom_hline(aes(yintercept = 0), colour = "black", linetype = "dashed") +
                      geom_hline(aes(yintercept = 1), colour = "gray66", linetype = "dashed") +
                      geom_hline(aes(yintercept = -1), colour = "gray66", linetype="dashed") +
                      theme(legend.position = 'none', text = element_text(size=20))
        volcano.plot <-   ggplot(df, aes(x = log2FoldChange, y = -log(padj, base = c(10)), group = significance_group, colour = significance_group)) +
                          geom_point() +
                          ggtitle('Volcano plot') +
                          xlab("M = log2(Fold Change)") + ylab("-log10(Adjusted P-Value)") + 
                          scale_color_manual(values = c("gray24", "red"), labels = legend_labels, name=lower_label) +
                          theme_bw() +
                          theme(legend.title = element_text(size = 16, face = "bold"), legend.text = element_text(size = 20),  text = element_text(size=20))
     grob <-   marrangeGrob(list(ma.plot,
                          volcano.plot),
                          top = textGrob(x, gp = gpar(fonsize = 40, fontface = 'bold', cex = 2), hjust = 1.8),
                          nrow = 1, ncol = 2,
                          widths=c(0.38, 0.62))
     invisible(grob)
    }
})

names(plots) <- contrast_names
```

```{r plotmavolStepwise, fig.keep = "all", fig.height = 10, fig.width = 20, echo=FALSE, results='asis'}

for(i in contrast_names){
  cat(paste0("\n\n\n##", i, "\n"))
  print(plots[[i]])
}

```


#Heatmaps


The first heatmap was made by hierarchically clustering the euclidean distances of the log2 fold change for genes with p-values less than `r pval` at any time point.

The second heatmap uses the same hierarchical method to cluster timepoints.


```{r pairwiseHeatmapsStepwise, results = "hide", fig.cap = "", fig.keep = "high"}


lfc_table <- all_results_tidyDF %>%
  mutate(significant = ifelse(dea_ID == 'padj' & dea_Value <= pval, yes = TRUE, no = FALSE)) %>% # label all significant genes
  group_by(contrastID, Gene) %>% # label the # of times genes that are significant
  ungroup()  %>%
  group_by(Gene) %>%
  filter(any(significant), dea_ID == 'log2FoldChange') %>%
  select(-significant) %>% unite(colname, contrastID, dea_ID) %>% # make the table wide formatted
  spread(colname, dea_Value)


## create a table of significant genes with the cluster colors assigned

sign.table <- all_results_tidyDF  %>% 
  spread(key = dea_ID, value = dea_Value)  %>% 
  filter(Gene %in% lfc_table$Gene) %>%
  group_by(contrastID, Gene) %>% 
  mutate(sortby = -log(padj, base = c(10))*sign(log2FoldChange)) %>% 
  ungroup() %>% gather(dea_ID, dea_Value, -Gene, -contrastID) %>% 
  unite(idAll, contrastID, dea_ID) %>% spread(key = idAll, value = dea_Value) %>%
  dplyr::rename('TranscriptID' = Gene)


heatmap.input.table           <- data.frame(lfc_table)
rownames(heatmap.input.table) <- lfc_table$Gene
heatmap.input.table$Gene      <- NULL
heatmap.input.table           <- na.omit(data.matrix(heatmap.input.table[which(rownames(heatmap.input.table) %in% sign.table$TranscriptID), ]))
colnames(heatmap.input.table) <- contrast_names


prettycolors  <- colorRampPalette(rev(brewer.pal(11, "Spectral")))
col.breaks    <- seq(-3, 3, length.out = ncol(heatmap.input.table))


## Make two heatmaps, allow custimizable graphing

hr <- hclust(dist(heatmap.input.table), method="complete") # Creates row dendrogram
hc <- hclust(dist(t(heatmap.input.table)), method="complete") # Creates column dendrogram

myclr     <- cutree(hr, k = 7) # set up where the groups will be, change 'k' for more or less groups
mycolr    <- c("Green", "Red", "Blue", "Orange", "Purple", "Pink", "Yellow") # must be the same length as 'k'
mycolr    <- mycolr[as.vector(myclr)] # pull out the colors assigned to each gene
mycolr.df <- data.frame(Cluster_Color = mycolr, TranscriptID = names(myclr)) # create a data frame of the cluster color each gene belongs to


if(ncol(heatmap.input.table) > 1){
    ## Make a heatmap without a column dendrogram
    col.breaks    <- seq(-3, 3, length.out = ncol(heatmap.input.table))
    heatmap       <- heatmap.2(heatmap.input.table, margin = c(15,5), col = prettycolors, dendrogram = 'row', trace = c("none"), Rowv=as.dendrogram(hr), Colv = NA, labRow = FALSE, key = TRUE, breaks = col.breaks, key.ylab = "Gene Count", key.xlab = "log2FoldChange", cexCol = 1.5, RowSideColors = mycolr)
    title(main = paste0("Clustering of ", nrow(heatmap.input.table)," Genes"), line = 0)
    par(cex.main=0.9)
}


if(ncol(heatmap.input.table) > 2) {
    k <- ifelse(ncol(heatmap.input.table < 5), 3, 5)
    myclc  <- cutree(hc, k = k) # create groupings for the column dendrogram
    mycolc <- c("Green", "Red", "Blue", "Orange", "Purple")
    mycolc <- mycolc[as.vector(myclc)]
    ## Make a heatmap of the logfold changes with column dendrogram.
    heatmap       <- heatmap.2(heatmap.input.table, margin = c(15,5), dendrogram = 'both', Rowv = as.dendrogram(hr), Colv = as.dendrogram(hc), col = prettycolors, trace = c("none"), labRow = FALSE, key = TRUE, breaks = col.breaks, main = paste0("Clustering of Timepoints on ", nrow(heatmap.input.table)," Genes"), key.ylab = "Gene Count", key.xlab = "log2FoldChange", cexCol = 1.5, RowSideColors = mycolr, ColSideColors = mycolc)
}

```



```{r sessionStepwise, echo=FALSE}
writeLines(capture.output(sessionInfo()), "sessionInfo/analysisStepwiseSessionInfo.txt")
```

#[R-session information](sessionInfo/analysisStepwiseSessionInfo.txt)


